The objective of this lab was to study and test the sordaria fimicola fungus crossover by determining what color it will yield during meiosis a cross over that will be between the wild type and the mutant alleles my hypothesis for this experiment is that all the mutant allele will appear in the. Carrie miller october 22, 2001 bios221 mw 11am sordaria fimicola introduction: in this lab we will be exploring the linkage and crossing of ascosporic color in sordaria by using tetrad analysis. Crossing over and gene conversion frequencies often differed significantly in a consistent direction between wild strains of the fungus sordaria fimicola isolated from a harsher or a milder microscale environment in “evolution canyon,” israel. Sordaria fimicola is one such dung fungus that has also long been used to study meiosis [1,2], and to teach genetics its products of meiosis, ascospores, form easily in artificial culture its products of meiosis, ascospores, form easily in artificial culture. Stoudt 1 jocie stoudt bio 110h sordaria lab report introduction sordaria fimicola reproduce through meiosis which consists of the phases prophase i, metaphase i, anaphase i, telophase i, prophase ii, metaphase ii, anaphase ii, and telophase ii.
For a class of 30 students culture the 3 sordaria strains and then set up cross plates of wild type x mutant tan and wild type x mutant gray the gene to centromere distance for tan and gray genes can then be calculated. Mapping genes in sordaria during the laboratory period when the sordaria cultures have matured, you will observe the asci under the compound microscope obtain a microscope slide place one drop of water in the center of the microscope slide. In a very similar laboratory experiment, meiosis and recombination in sordaria fimicola, the same approaches of the two labs shared common procedures including: crossing a wild type and mutant type gene, growing the hyphae in rotting vegetation, and calculating the genetic map distances.
The sfs receives multiple times the uv radiation from the sun that the nfs receives, has fluctuating weather, and is generally an arid and less hospitable region compared to the temperate, stable environment of the nfs scientists collected wild type sordaria fimicola inhabiting both slopes and discovered that the strains from the sfs exhibited higher [. In another lab project i am considering for the fall class, i have been experimenting with crossing sordaria fimicola fungi these are molds in the huge phylum of ascomycete fungi that have spores in filaments (), which are ordered meiotic products so it is an excellent example of meiosis and genetic recombination--if you can get it to work. Sordaria fimicola is an ascomycete fungus that can be used to demonstrate the results of crossing over during meiosis sordaria is a haploid organism for most of its life cycle. Sordaria lab report the effect of environmental conditions on the crossover frequency in sordaria fimicola introduction the general consensus of recombination and genetic crossovers.
In this video paul andersen shows you how a cross between mutant and wild types of sordaria fimicola can be used to show and measure frequency of crossing-over. Lab 10 – meiosis and tetrad analysis objectives: explain how meiosis and crossing over result in the different arrangements of lab 10 page 2 of 10 sordaria fimicola is an ascomycete fungus that can be used to demonstrate the results of crossing over during meiosis. Where he is coordinator of the introductory biology laboratory course for biology majors he received his bs in biology (1967) and ph d in behavioral ecology (1971) from we selected the genus sordaria over neurospora for this study because of the absence of asexual, in sordaria fimicola (figure 11),.
Meiosis and genetic diversity in sordaria fimicola haley demartin lab section 001 due october 27, 2014 introduction several evolution canyons exist in lower nahal oren, mount carmel, israel. Sordaria lab effects of crossing over in sordaria fimicola abstract sexual reproduction in organisms is a cause for genetic variation this can be seen through the process of meiosis in sordaria fimicola because of the effects of crossing over and independent assortment that occur in meiosis i. Sordaria can be used as a model to study meiotic segregation the trait followed was the ascospore color ascospore color is a single gene trait therefore it is easily observed under a light microspore. In the sordaria genetics advanced inquiry lab kit for ap biology, prepare cross plates and cross wild-type and mutant strains of sordaria fimicolaobserve the life cycle of a member of the fungal kingdom.
Flipped lab videos 2,072 views 10:15 planted digital learning library - gram stain procedure - duration: sordaria primicola and crossing over - duration: 10:16 john croteau 3,677 views. View notes - lab report - rough draft(2) from biol 1162 at university of houston mapping the crossing over genes for spore in the fungus sordaria nikhil thomas, april 8, 2008, lon ridgway abstract. Sordaria lab pictures sordaria octad below you will find reproductions of a number of sordaria octad preparations for the purposes of mapping the tan locus with respect to its centromere, count only complete tetrads and count all complete tetrads on a particular slide. Chart and diagram slides for powerpoint - beautifully designed chart and diagram s for powerpoint with visually stunning graphics and animation effects our new crystalgraphics chart and diagram slides for powerpoint is a collection of over 1000 impressively designed data-driven chart and editable diagram s guaranteed to impress any audience.
Sordaria lab ap biology lab investigation 7/meiosis helpful site: sordaria. Sordaria fimicola is a species of microscopic fungus it is commonly found in the feces of herbivores sordaria fimicola is often used in introductory biology and mycology labs because it is easy to grow on nutrient agar in dish cultures. Understanding sordaria while i have done the sordaria lab for many years, i don’t think that i really understood it until 2017here goes sordaria fimicola is a fungus with a lifestyle that gives us a window into meiosis and crossing over. Introduction sordaria fimicola is a fungi that is a part of the ascomycota phylum and is a model organism organisms in this phylum have a reproductive characteristic called an ascus and the parethecium ( a sac-like cell containing 4 to 8 haploid spores ) contains the ascus these spores are produced by meiosis to make 4 haploid spores.
1 demonstration of crossing-over during meiosis in sordaria fimicola introduction sexual reproduction is a special case of cell reproduction in which the genes of two. Sordaria lab report the sf receives multiple times the iv radiation from the sun that the naps receives, has fluctuating weather, and is generally an arid and sees hospitable region compared to the temperate, stable environment of the nfg. Having lab strains of sordaria fimicola makes it a good model organism because it has a short life cycle, produces fruiting bodies, and it’s easy to keep alive the defining characteristic of the phylum ascomycota is the production of four to eight.